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immunogenic sequence reeklrnqrrqasntpshi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immunogenic sequence reeklrnqrrqasntpshi
    Immunogenic Sequence Reeklrnqrrqasntpshi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunogen+sequence/immunogenic+sequence+reeklrnqrrqasntpshi/pmc11364236-87-18-16
    Average 90 stars, based on 1 article reviews
    immunogenic sequence reeklrnqrrqasntpshi - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early–Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 °C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat Anti-Rabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early-Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study.
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 ◦C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat AntiRabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Incubation:

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early–Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 °C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat Anti-Rabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early-Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study.
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 ◦C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat AntiRabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Sequencing:

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early–Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 °C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat Anti-Rabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Article Title: The Profile of Selected Protein Markers of Senescence in the Placentas of Cows During Early-Mid-Pregnancy and Parturition with and Without the Retention of Fetal Membranes: A Preliminary Study.
    Article Snippet: Membranes were blocked with Animal-Free BlockerTM 5X Concentrate (SP-5030, Vector Labs, Janki, Poland) in PBS with 0.05% Tween (PBST) for 1 h at room temperature. .. After blocking and discarding the solution, membranes were incubated overnight at 4 ◦C with primary antibodies diluted in PBST: p21 monoclonal antibody (1:500, MA5-31479, Thermo ScientificTM, Rockford, IL, USA, at least 83% immunogen sequence identity with cow), p53 monoclonal antibody (1:500, MA5-12557, Thermo ScientificTM, Rockford, IL, USA), p38 MAPK alpha polyclonal antibody (1:3334, PA5-34769, Thermo ScientificTM, Rockford, IL, USA) and phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (1:500, 36-8500, Thermo ScientificTM, Rockford, IL, USA). .. After incubation, membranes were washed 3 times for 10 min each in PBST and then incubated for 2 h at room temperature with secondary antibodies diluted in PBST: goat AntiRabbit IgG (1:1333, 31346, Thermo ScientificTM, Rockford, IL, USA), or goat Anti-Mouse IgG (1:1333, 31321, Thermo ScientificTM, Rockford, IL, USA), conjugated with alkaline phosphatase.

    Article Title: The CatSper channel is present and plays a key role in sperm motility of the Atlantic salmon (Salmo salar).
    Article Snippet: Among all the Ca channels, CatSper channels have been one of the most studied in sperm of different species due to their demonstrated role in the fertilization process.. In fish sperm, the calcium channel plays a key role in sperm activation.. However, the functionality of the CatSper channels has not been studied in any of the fish species.

    other:

    Article Title: Developmental Regulation and Specific Brain Distribution of Phosphorabphilin
    Article Snippet: A peptide with unrelated sequence, a peptide with the same sequence but with unphosphorylated serine (related nonphosphopeptide), and the peptide used as immunogen (phosphopeptide) were coupled to Imject maleimide-activated bovine serum albumin (BSA; Pierce).

    Article Title: Mutations in ACTRT1 and its enhancer RNA elements lead to aberrant activation of Hedgehog signaling in inherited and sporadic basal cell carcinomas.
    Article Snippet: For the study of ARP-T1 recruitment to the GLI1 promoter, DNA fragments were immunoprecipitated with anti-ARP-T1 (PA5-31691, immunogen sequence residues 174–376, Thermo Scientific), anti–RNA Pol II and IgG antibodies.

    Article Title: Serotonergic modulation of swallowing in a complete fly vagus nerve connectome.
    Article Snippet: For 5-HT/Trhn staining, primary antibodies were anti-5HT (1:1000, rabbit, Sigma-Aldrich, S5545) and anti-Trhn (1:250, guinea pig, generated by Thermo, immunogen sequence: DSFEEAKEQMRAFAESIQR), secondary antibodies were anti-rabbit Alexa Fluor 633 (1:500, goat, Invitrogen, A-21071) and antiguinea pig Alexa Fluor 633 (1:500, goat, Invitrogen, A-21105).



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    (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the <t>immunogenic</t> sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.
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    Image Search Results


    (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

    Journal: PLOS ONE

    Article Title: PAX6 protein in neuromasts of the lateral line system of salamanders ( Eurycea )

    doi: 10.1371/journal.pone.0293163

    Figure Lengend Snippet: (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

    Article Snippet: Primary antibody was diluted 1:10,000 in 5% non-fat dry milk in Tris-buffered saline with Tween20 (TBST), and half was incubated with custom peptide (Thermo Fisher) matching the antibody’s immunogenic sequence, resuspended in PBS at a concentration at least 5 times the molarity of the antibody.

    Techniques: Sequencing